Plasmid_Backbone

Part:BBa_K4202016

Designed by: ZIHANG YE   Group: iGEM22_ZJU-China   (2022-10-01)


PHY-300PLK is a Escherichia coli-Bacillus subtilis shuttle vector

PHY300PLK is one of the smallest hybrids of a new series of chimeric plasmids using the parental plasmids, pACYC177 of E.coli and pAM-alpha of Streptococcus faecalis. This shuttle vector for E.coli and B.subtilis contains an RNA primer gene for ori-177; the R-Tc gene; the R-Ap gene; Rep-alpha-1 gene, which is the plasmid replication gene; two replication origins, one for E.coli and the other for B. subtilis; and a polylinker. In the project of iGEM22_ZJU-China, we utilize this plasmid for the protein expression in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 37
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 3830
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 37
    Illegal PstI site found at 15
    Illegal PstI site found at 3830
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 37
    Illegal BglII site found at 11
    Illegal BamHI site found at 27
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 37
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 3830
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 37
    Illegal XbaI site found at 6
    Illegal PstI site found at 15
    Illegal PstI site found at 3830
    Illegal AgeI site found at 4709
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2587
    Illegal BsaI site found at 4004


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Categories
Parameters
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