Plasmid_Backbone
Part:BBa_K4202016
Designed by: ZIHANG YE Group: iGEM22_ZJU-China (2022-10-01)
PHY-300PLK is a Escherichia coli-Bacillus subtilis shuttle vector
PHY300PLK is one of the smallest hybrids of a new series of chimeric plasmids using the parental plasmids, pACYC177 of E.coli and pAM-alpha of Streptococcus faecalis. This shuttle vector for E.coli and B.subtilis contains an RNA primer gene for ori-177; the R-Tc gene; the R-Ap gene; Rep-alpha-1 gene, which is the plasmid replication gene; two replication origins, one for E.coli and the other for B. subtilis; and a polylinker. In the project of iGEM22_ZJU-China, we utilize this plasmid for the protein expression in Bacillus subtilis.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 37
Illegal XbaI site found at 6
Illegal PstI site found at 15
Illegal PstI site found at 3830 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 37
Illegal PstI site found at 15
Illegal PstI site found at 3830 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 37
Illegal BglII site found at 11
Illegal BamHI site found at 27 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 37
Illegal XbaI site found at 6
Illegal PstI site found at 15
Illegal PstI site found at 3830 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 37
Illegal XbaI site found at 6
Illegal PstI site found at 15
Illegal PstI site found at 3830
Illegal AgeI site found at 4709 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 2587
Illegal BsaI site found at 4004
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Categories
Parameters
None |